■ 基本信息
啟動(dòng)子: | PC,polyhedrin |
復制子: | pUC ori，F1 ori |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 廣宿主系列，昆蟲(chóng)細胞載體 |
質(zhì)粒大小: | 4858bp |
質(zhì)粒標簽: | N-His；N-TEV |
原核抗性: | Amp |
真核抗性: | Gen |
克隆菌株: | DH5α |
培養條件: | 37℃，有氧 LB |
表達宿主: | 昆蟲(chóng)細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3'測序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK2327pFastBacHTC昆蟲(chóng)胞內質(zhì)粒.txt
