■ 基本信息
啟動(dòng)子: | H1 promoter |
復制子: | pBR322 ori，SV40 ori |
質(zhì)粒大小: | 4555bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
|
片段物種: |
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原核抗性: | Amp |
真核抗性: | Hyg
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pSilencer3.1-H1-Stuffer-Hygro是一個(gè)哺乳細胞干擾載體，通過(guò)BamHI和HindIII酶切位點(diǎn)可連入SiRNA，點(diǎn)擊查看本質(zhì)粒的參考資料：
pSilencer hygro Kit.pdf.（點(diǎn)擊查看）
The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1717 pSilencer3.1-H1-Stuffer-Hygro哺乳干擾質(zhì)粒.txt
