■ 基本信息
別名: | 四環(huán)素激活 |
啟動(dòng)子: | Tet |
復制子: | pUC |
質(zhì)粒分類(lèi): | 慢病毒表達質(zhì)粒 |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
5'測序引物: | 根據全序列設計 |
3'測序引物: | 根據全序列設計 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF |
片段物種: |
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標記: |
|
■ 質(zhì)粒簡(jiǎn)介
慢病毒載體pLVX-TetOne-Puro是集調控與應答功能于一體的四環(huán)素誘導載體。The Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing mammalian cells. The all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. In addition, this vector contains a puromycin antibiotic selection marker.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1692 pLVX-TetOne-Puro慢病毒四環(huán)素激活表達質(zhì)粒.txt
