■ 基本信息
別名: | Plasmid #22733 |
啟動(dòng)子: | PGK |
復制子: | ori，F1 ori |
終止子: | bGH poly(A) signal |
質(zhì)粒分類(lèi): | 基因文庫質(zhì)粒；其它基因質(zhì)粒；其它種源質(zhì)粒 |
質(zhì)粒大小: | 6678bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | DH5a |
培養條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 基因編輯 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.
pLCA.66/2272質(zhì)粒的參考文獻：Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1548 pLCA.662272同源重組質(zhì)粒.txt
