■ 基本信息
啟動(dòng)子: | gpdA |
復制子: | pUC |
終止子: | trpC terminator |
質(zhì)粒分類(lèi): | 絲狀真菌 |
質(zhì)粒大小: | 5518bp |
質(zhì)粒標簽: | 無(wú) |
原核抗性: | Amp |
篩選標記: | Bar |
克隆菌株: | DH5α |
培養條件: | 37℃ |
表達宿主: | 絲狀真菌 |
誘導方式: | 無(wú)需誘導 |
5'測序引物: | 根據全序列設計 |
3'測序引物: | 根據全序列設計 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 絲狀真菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | 基因CDS
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pBARGPE1是一個(gè)絲狀真菌-大腸桿菌穿梭表達載體，利用gpdA promoter可啟動(dòng)外源目的基因的表達，轉化至絲狀真菌中可用草胺膦Bar篩選。The plasmid carries a polylinker with 5 or 6 unique restriction sites flanked on the right by a fungal promoter directing transcription into the polylinker and flanked on the left by the trpC terminator. The plasmid carry a bar selectable marker and a lox-NotI-lox site for insertion into lambda and for site-specific recombination between lox sites.
pBARGPE1質(zhì)粒的參考文獻：Infection cycle of maize stalk rot and ear rot caused by Fusarium verticillioides.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1063pBARGPE1真菌表達質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
