■ 基本信息
別名: | pFastBac Dual |
啟動(dòng)子: | P10+PH |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 廣宿主系列，昆蟲(chóng)細胞載體 |
質(zhì)粒大小: | 5238bp |
原核抗性: | Amp |
真核抗性: | Gen |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 昆蟲(chóng)細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'測序引物: | pFastBac-R:ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 多框空載
|
原核抗性: | Amp,Gen |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pFastBac Dual昆蟲(chóng)細胞表達載體包含有兩個(gè)啟動(dòng)子，能夠同時(shí)表達兩個(gè)外源基因，一個(gè)表達基因是polyhedrin (PH)啟動(dòng)子控制，另一個(gè)外源基因是由p10啟動(dòng)子控制。
pFastBacDual 載體的特點(diǎn)是在單個(gè)載體上有兩個(gè)啟動(dòng)子，用于在使用 Bac-to-Bac? 桿狀病毒表達系統(Bac-to-Bac? Baculovirus Expression System) 時(shí)在昆蟲(chóng)細胞中同時(shí)表達兩個(gè)蛋白質(zhì)。該載體具有兩個(gè)強啟動(dòng)子，多角體蛋白啟動(dòng)子和p10 啟動(dòng)子，因此可實(shí)現高水平表達。包含 CAT和β-葡萄糖醛酸酶 (gus) 的表達對照。
The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:
Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac? E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1045pFastBacDual昆蟲(chóng)雙框質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
