■ 基本信息
啟動(dòng)子: | PH |
復制子: | pUC |
終止子: | polyhedrin 3 |
質(zhì)粒分類(lèi): | 昆蟲(chóng)細胞載體；桿狀病毒組成型分泌表達質(zhì)粒 |
質(zhì)粒大小: | 9761bp |
質(zhì)粒標簽: | N-GP67 signal |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 昆蟲(chóng)細胞 |
誘導方式: | 組成性表達，無(wú)需誘導 |
5'測序引物: | Polyhedrin-F：AAATGATAACCATCTCGC |
3'測序引物: | Polyhedrin-R：GTCCAAGTTTCCCTG |
備注: | 含有GP67分泌信號肽 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The acidic glycoprotein gp67 (syn.: gp64) is the most abundant envelope surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV baculovirus), and is essential for the entry of baculovirus particles into susceptible insect cells. Since large amounts of this protein are secreted and anchored to the virus peplomer, its gene contains one of the most effective baculovirus-encoded signal sequences for protein secretion. Therefore, we have constructed baculovirus transfer vectors that contain the gp67 signal sequence in front of a multiple cloning site. A gene of choice can be inserted in one of these cloning sites and the protein of interest will be expressed as a gp67 signal peptide fusion protein under the control of the strong baculovirus polyhedrin promoter. This strategy allows the forced secretion of otherwise non-secreted recombinant proteins which may be easily purified when serum-free insect culture medium, BD BaculoGold Max-XP Insect Cell Medium is used. The transfer vector(s) should be preferentially used in conjunction with BD BaculoGold DNA .
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1044pAcGP67A昆蟲(chóng)分泌質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
