■ 基本信息
啟動(dòng)子: | PH |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 廣宿主系列,昆蟲(chóng)細胞載體 |
質(zhì)粒大小: | 4857bp |
質(zhì)粒標簽: | N-His;N-TEV |
原核抗性: | Amp |
真核抗性: | Gen |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 昆蟲(chóng)細胞 |
誘導方式: | 無(wú)須誘導,瞬時(shí)表達 |
5'測序引物: | pFastbac-F:TATTCCGGATTATTCATACC |
3'測序引物: | pFastBac-R:ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲(chóng)細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載:
ZK1039pFastBacHTB昆蟲(chóng)胞內質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確,不保證表達效果。