■ 基本信息
別名: | pHT-304 |
啟動(dòng)子: | Pgrac |
復制子: | pUC |
質(zhì)粒分類(lèi): | 廣宿主系列，枯草桿菌載體 |
質(zhì)粒大小: | 6530bp |
質(zhì)粒標簽: | LacZa |
原核抗性: | Amp |
真核抗性: | Erm |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 枯草芽孢桿菌 |
誘導方式: | IPTG誘導 |
5'測序引物: | M13R:CAGGAAACAGCTATGACC |
3'測序引物: | M13F:TGTAAAACGACGGCCAGT |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 枯草桿菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The promoter of pHT304 vector is Lac, which can be used to induce the expression of recombinant protein in Escherichia coli DE3 engineering bacteria (DE3 BL21 or DE3 Rosetta). The tag has LacZa, which can be purified by nickel column.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1012pHT304枯草胞內質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
