■ 基本信息
啟動(dòng)子: | ADH1 |
復制子: | 2μ |
質(zhì)粒分類(lèi): | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 5524bp |
原核抗性: | Amp |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | 根據序列設計引物 |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | TRP1
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pGBT9 generates a hybrid protein that contains the sequences for the GAL4 DNA-binding domain (DNA-BD; a.a. 1–147). For the construction of a hybrid protein, the gene encoding the protein of interest is ligated into the MCS in the correct orientation and in the correct reading frame such that a fusion protein is generated. The fusion protein is expressed in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that are an intrinsic part of the GAL4 DNA-BD . pGBT9 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae. It carries the bla gene (for ampicillin resistance in E. coli) and the TRP1 nutritional marker that allow yeast auxotrophs carrying pGBT9 to grow on limiting synthetic medium lacking Trp.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK983pGBT9酵母雙雜交質(zhì)粒.txt
