■ 基本信息
啟動(dòng)子: | ADH1 |
復制子: | 2μ |
質(zhì)粒分類(lèi): | 酵母系列，酵母雙雜交載體 |
質(zhì)粒大小: | 7.9kb |
原核抗性: | Kan |
真核抗性: | TRP1 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 酵母菌 |
質(zhì)粒用途: | 雜交 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | TRP1
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測序為準）下載：
ZK982 pGBKT7-lam酵母雙雜交質(zhì)粒.txt
