■ 基本信息
啟動(dòng)子: | 35s |
復制子: | pUC |
終止子: | NOS |
質(zhì)粒分類(lèi): | 植物系列，RNAi載體 |
質(zhì)粒大小: | 12880bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 植物細胞 |
5'測序引物: | 35S:GACGCACAATCCCACTATCC |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA |
片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
siRNA) caused by RNA (interference RNA short)Interference (RNAi) is a very effective test method. It is a creature The body of a molecule by double stranded RNA (dsRNA) molecules to make specific Degradation of the sequence of mRNA resulted in the silencing of gene expression. This phenomenon occurs at the post transcriptional level, that is, sequence specific Post transcriptional gene silencing.
