■ 基本信息
啟動(dòng)子: | 35s |
復制子: | ori |
終止子: | NOS |
質(zhì)粒分類(lèi): | 植物系列，蛋白過(guò)表達載體 |
質(zhì)粒大小: | 12880bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
5'測序引物: | 35S:GACGCACAATCCCACTATCC |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
An intron sequence of Poptr;CYCD1;1 from Populus trichocarpa was ligated into binary vector,pROKⅡ, to obtain the construct for gene silencing in plants. The resulting pRNAi was used to silence the exogenous fusion gene, GUS:GFP, in transgenic tobacco transformed with pCAMBIA-1303 vector. The specific sequence from GUS and GFP genes was cloned into pRNAi, respectively, and both of them reduced the expression of GUS:GFP gene. The results implied that the present pRNAi could be used to silence some gene of interest.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK848pROKII植物表達質(zhì)粒.txt
