■ 基本信息
別名: | Plasmid #8454;VSVG |
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | β-globin poly(A) |
質(zhì)粒分類(lèi): | 病毒系列，慢病毒包裝載體 |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 輔助包裝 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus (VSV-G) under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus (MMLV)-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipid components of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK837pCMV-VSV-G慢病毒包裝質(zhì)粒.txt
