■ 基本信息
啟動(dòng)子: | Ubc |
復制子: | pUC |
終止子: | SV40 poly(A) signal，bGH poly(A) signal |
質(zhì)粒分類(lèi): | 病毒系列，慢病毒干擾載體 |
質(zhì)粒大小: | 13320bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA |
片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The Thermo Scientific Open Biosystems TRIPZ Lentiviral Inducible shRNAmir Library was developed in collaboration with Dr. Greg Hannon (CSHL) and Dr. Steve Elledge (Harvard). This library combines the design advantages of microRNA-adapted shRNA (shRNAmir) with the pTRIPZ lentiviral inducible vector to create a powerful RNAi trigger capable of producing RNAi in most cell types. The vector is engineered to be Tet-On and produces tightly regulated induction of shRNAmir expression in the presence of doxycycline.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK821pTRIPZ慢病毒干擾質(zhì)粒.txt
