■ 基本信息
啟動(dòng)子: | U6 |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 病毒系列，慢病毒干擾載體 |
質(zhì)粒大小: | 7539bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| 根據序列設計引物 |
3'測序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
|
片段物種: |
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原核抗性: | Amp |
真核抗性: | Hyg
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pLKO.1 hygro is a replication-incompetent lentiviral vector for expression of shRNAs. pLKO.1 hygro can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line. Once introduced, the hygromycin resistance marker encoded in pLKO.1 allows for convenient stable selection.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK813pLKO.1-Hygro慢病毒干擾質(zhì)粒.txt
