■ 基本信息
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 病毒系列，慢病毒克隆質(zhì)粒 |
質(zhì)粒大小: | 8102bp |
原核抗性: | Amp |
篩選標記: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37℃ |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞,慢病毒 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | 嘌呤霉素
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK756pLVX-Puro慢病毒表達質(zhì)粒.txt
