■ 基本信息
啟動(dòng)子: | Lac |
復制子: | pUC |
質(zhì)粒分類(lèi): | 哺乳細胞，蛋白過(guò)表達載體 |
質(zhì)粒大小: | 4392bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | M13R:CAGGAAACAGCTATGACC |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 輔助包裝 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The use of pAdVAntage vectors were transfected into mammalian cells can promote the initiation of translation to enhance the instantaneous protein expression in many cell types.
Transfection of mammalian cells with expression vector often leads to the expression of the target protein. The double stranded RNA (dsRNA), which is produced in the transfection process, is thought to activate the dsRNA- activation inhibitor (DAI), which is one of the many kinds of enzymes involved in the host cell antiviral defense system. DAI phosphorylation of translation initiation factor eIF-2, termination of translation and generation of protein. However, with the pAdVAntage vector were transfected into DAI in the process of translation inhibition by RNA polymerase III generated adeno-associated virus type I (RNA VAI RNA) offset. RNA DAI combined with VAI to prevent its activation, so as to complete the translation and protein expression.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK706pAdVAntage哺乳輔助質(zhì)粒.txt
