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    產(chǎn)品名稱(chēng):pTRE2pur

    貨號 規格 價(jià)格 訂購數量 是否現貨
    ZK692 1μg(20μl,50ng/μl) 870 - + 有貨

    基本信息

    啟動(dòng)子:

    TRE

    復制子:

    pUC

    終止子:

    SV40 poly(A) signal,beta-globin polyA signal

    質(zhì)粒分類(lèi):

    哺乳細胞,四環(huán)素調控系統載體

    質(zhì)粒大小:

    5108bp

    原核抗性:

    Amp

    克隆菌株:

    DH5a

    培養條件:

    37度

    表達宿主:

    哺乳細胞

    誘導方式:

    四環(huán)素誘導

    5'測序引物:

    Sv40-polyA-R:GAAATTTGTGATGCTATTGC

    3'測序引物:

    根據序列設計引物


    質(zhì)粒屬性

    質(zhì)粒宿主:

    哺乳細胞

    質(zhì)粒用途:

    蛋白表達

    片段類(lèi)型:


    片段物種:


    原核抗性:

    Amp

    真核抗性:

    熒光標記:



    質(zhì)粒簡(jiǎn)介

    pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On? and Tet-Off? Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.


    質(zhì)粒圖譜


    質(zhì)粒序列

    質(zhì)粒序列請下載:ZK692pTRE2pur哺乳調控質(zhì)粒.txt

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    總價(jià)格:¥2000
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