■ 基本信息
別名: | pSilencer2.1-U6 neo |
啟動(dòng)子: | U6 |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細胞，信號通路報告載體 |
質(zhì)粒大小: | 4521bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | U6:ATGGACTATCATATGCTTACCGTA |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
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片段物種: |
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原核抗性: | Amp |
真核抗性: | G418
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
This Ambion vector is for the expression of siRNA. It has an antibiotic resistance gene (neomycin) to enable selection of transfected cells and features the human RNA polymerase III promoter (U6). Sufficient reagents are provided for 20 reactions.
■ 質(zhì)粒圖譜
