■ 基本信息
別名: | 人源MAP1LC3B |
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒大小: | 5113bp |
質(zhì)粒標簽: | N-EGFP |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞293T |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物:
| Sv40-polyA-R：GAAATTTGTGATGCTATTGC
|
備注: | 人源基因哺乳細胞過(guò)表達質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 人
|
原核抗性: | Kan |
真核抗性: | G418
|
熒光標記: | 綠色
|
■ 質(zhì)粒簡(jiǎn)介
pEGFP-LC3b質(zhì)粒是一個(gè)哺乳細胞過(guò)表達質(zhì)粒，CMV啟動(dòng)子驅動(dòng)EGFP綠色熒光蛋白基因和LC3b人源基因融合表達，可用于細胞自噬研究。本質(zhì)粒的參考文獻共有三篇，分別是：
1.Crosstalk of ROS/RNS and autophagy in silibinin-induced apoptosis of MCF-7 human breast cancer cells in vitro.
2.Type I collagen promotes primary cilia growth through down-regulating HDAC6-mediated autophagy in confluent mouse embryo fibroblast 3T3-L1 cells.
3.Silibinin negatively contributes to primary cilia length via autophagy regulated by histone deacetylase 6 in confluent mouse embryo fibroblast 3T3-L1 cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK535pEGFP-LC3b哺乳自噬質(zhì)粒.txt
