■ 基本信息
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細胞，熒光蛋白報告載體 |
質(zhì)粒大小: | 4793bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| 根據序列設計引物
|
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 定位 |
片段類(lèi)型: |
|
片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
|
熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
pEYFP-Mem encodes a fusion protein consisting of the N-terminal 20 amino acids of neuromodulin,also called GAP-43, and a yellow-green fluorescent variant of the enhanced green fluorescent protein (EGFP). The neuromodulin fragment contains a signal for posttranslational palmitoylation of cysteines 3 and 4 that targets EYFP to membranes. The EYFP gene contains four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm, and the emission maximum is 527 nm (in the yellow-green region).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK530pEYFP-Mem細胞膜定位質(zhì)粒.txt
