■ 基本信息
別名: | 有背景;Plasmid #22010 |
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | hGH poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細胞，雙分子熒光互補載體 |
質(zhì)粒大小: | 5216bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 信號報告 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
BiFC-VN173 by pFlag-CMV Xba I and BamHI carrier in the site of insertion of the VN173 gene, the plasmid is high copy plasmid, VN173 gene derived from Vitoria victoria. Visualization of Aequorea Aequorea ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK517pBiFC-VN173雙分熒光質(zhì)粒.txt
