■ 基本信息
啟動(dòng)子: | SV40 |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細胞，信號通路報告載體 |
質(zhì)粒大小: | 4879bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37℃ |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| RVP3:CTAGCAAAATAGGCTGTCCC
|
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 信號報告 |
片段類(lèi)型: | Promoter
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標記: | Rluc
|
■ 質(zhì)粒簡(jiǎn)介
The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK486pGL4.79[hRluc-Neo]哺乳報告質(zhì)粒.txt
