■ 基本信息
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細胞，熒光蛋白報告載體 |
質(zhì)粒大小: | 4722bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | mCherry-R:TTGGTCACCTTCAGCTTGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Kan |
真核抗性: | G418
|
熒光標記: | 紅色
|
■ 質(zhì)粒簡(jiǎn)介
pmCherry-N1 is a mammalian expression vector designed to express a protein of interestfused to the N-terminus of mCherry, a mutant fluorescent protein derived from thetetrameric Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively. Expression of fusion proteins that retain the fluorescent properties of the unmodified mCherry protein can be monitored by flow cytometry and their localization in vivo can be determined by fluorescence microscopy.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK437pmCherry-N1哺乳熒光質(zhì)粒.txt
