■ 基本信息
啟動(dòng)子: | CAG |
復制子: | pUC |
終止子: | β-globin poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳系列質(zhì)粒；哺乳表達質(zhì)粒；pCAG系列質(zhì)粒 |
質(zhì)粒大小: | 4801bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37℃，5%CO2 |
表達宿主: | 293T等哺乳細胞 |
誘導方式: | 無(wú)需誘導，瞬時(shí)表達 |
5'測序引物: | pcaggs-F（GTTCGGCTTCTGGCGTGT） |
3'測序引物: | pcaggs-R（TATGTCCTTCCGAGTGAGAG） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
該質(zhì)?？捎糜谠陔ub-肌動(dòng)蛋白，兔b-球蛋白雜合啟動(dòng)子（CAG）的控制下高效表達基因，和各種哺乳動(dòng)物細胞中的人CMV-IE增強子; CAG啟動(dòng)子序列由雞b-肌動(dòng)蛋白啟動(dòng)子，第一外顯子和第一內含子的一部分（似乎具有強的增強子樣活性），與兔b-珠蛋白片段連接，由第二內含子的3'部分 包括正常拼接反應所需的分支點(diǎn)）和第三外顯子的5'部分。
This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK399pCAGGS哺乳表達質(zhì)粒.txt
