■ 基本信息
別名: | pCDNA3.1(-)-Myc-HisB |
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | BGH poly（A）signal |
質(zhì)粒分類(lèi): | 哺乳細胞載體;蛋白過(guò)表達質(zhì)粒 |
質(zhì)粒大小: | 5520bp |
質(zhì)粒標簽: | 6X His, myc |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: | G418 |
熒光標記: |
|
■ 質(zhì)粒簡(jiǎn)介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK358pCDNA3.1-Myc-HisB(-)哺乳表達質(zhì)粒.txt
