■ 基本信息
別名: | pCDNA3 |
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | bGH poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳系列質(zhì)粒；哺乳表達質(zhì)粒；pCDNA系列質(zhì)粒 |
質(zhì)粒大小: | 5446bp |
質(zhì)粒標簽: | 無(wú) |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37℃，5%CO2 |
表達宿主: | 293T等哺乳細胞 |
誘導方式: | 無(wú)需誘導 |
5'測序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'測序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
備注: | 常用的哺乳細胞真核表達載體 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418 |
熒光標記: |
|
■ 質(zhì)粒簡(jiǎn)介
pCDNA3.0載體是專(zhuān)門(mén)設計用來(lái)在哺乳細胞中進(jìn)行高水平的穩定表達和瞬時(shí)表達。其中人巨細胞病毒早期啟動(dòng)子（CMV）在廣泛的哺乳動(dòng)物細胞中可以實(shí)現高表達。另外為篩選穩定細胞系提供了新霉素抗性基因，可用G418篩選。
pcDNA3.0 is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells. Neomycin resistance gene for selection of stable cell lines.
pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
特征片段：
CMV promoter: bases 209-863 T7 promoter: bases 864-882
Polylinker: bases 889-994 Sp6 promoter: bases 999-1016
BGH poly A: bases 1018-1249 SV40 promoter: bases 1790-2115
SV40 origin of replication: bases 1984-2069 Neomycin ORF: bases 2151-2945
SV40 poly A: bases 3000-3372 ColE1 origin: bases 3632-4305
Ampicillin ORF: bases 4450-5310
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK344pCDNA3.0哺乳表達質(zhì)粒.txt
