■ 基本信息
別名: | pET302NTHis |
啟動(dòng)子: | T7 |
復制子: | pBR322 |
終止子: | T7 terminator |
質(zhì)粒分類(lèi): | 大腸桿菌載體；PET系列表達質(zhì)粒 |
質(zhì)粒大小: | 5712bp |
質(zhì)粒標簽: | N-6×His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37℃，有氧，LB |
表達宿主: | BL21(DE3) |
誘導方式: | IPTG或乳糖及其類(lèi)似物 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The Champion pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia E. coli Expression Medium.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK192pET302NT-His大腸表達質(zhì)粒.txt
