■ 基本信息
啟動(dòng)子: | SV40，T7，T3 |
復制子: | pBR322 |
質(zhì)粒分類(lèi): | 哺乳細胞，蛋白過(guò)表達載體 |
質(zhì)粒大小: | 7.9kb |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: | G418
|
熒光標記: |
|
■ 質(zhì)粒簡(jiǎn)介
SuperCos 1 is a novel, 7.9-kb cosmid vector that contains bacteriophage promoter sequences flanking a unique cloning site (see Figure 1). This structure allows rapid synthesis of "walking" probes specific for the extreme ends of insert DNA. The SuperCos 1 vector is also engineered to contain genes for the amplification and expression of cosmid clones in eukaryotic cells. In addition, most genomic inserts can be excised as a single large restriction fragment using the Not I restriction site that flanks the SuperCos 1 polylinker.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達效果。
■ 質(zhì)粒圖譜
